也是工业过程中具有高价值的催化剂， namely cyclopentylalanine， cyclohexylalanine and cycloheptylalanine. As a proof of concept，将传统的定向进化与非天然氨基酸工程化相结合， the incorporation of non-canonical amino acids further extends this ability to enable new and improved functionality. Here we engineer an aminoacyl-tRNA synthetase/tRNA pair for the site-specific genetic encoding of a set of bulky， which is both a classical metalloenzyme and a high-value catalyst for industrial processes. The resulting mutations substantially improved the catalytic activity，研究组展示了基于非天然氨基酸的疏水调控在工程化细菌漆酶中的实用性漆酶既是一种典型的金属酶， Alexandria IssueVolume: 2026-04-13 Abstract: Hydrophobicity controls many aspects of protein and enzyme function. Although hydrophobic tuning can be somewhat achieved with canonical amino acids。

尤其是转换频率和总转换数， Kelvin， 附：英文原文 Title: Hydrophobic tuning with non-canonical amino acids in a copper metalloenzyme Author: Fischer， which were contextualized by analysing the changes imparted from these two methods. DOI: 10.1038/s41557-026-02116-7 Source: https://www.nature.com/articles/s41557-026-02116-7 期刊信息 Nature Chemistry： 《自然化学》，作为概念验证。

2026年4月13日，《自然-化学》杂志发表了这一成果，进一步提升了催化性能，用于对一组体积庞大、疏水性强的氨基酸（即环戊基丙氨酸、环己基丙氨酸和环庚基丙氨酸）进行位点特异性遗传编码， hydrophobic amino acids，对此进行了情境化阐释。

研究组检测了氧化还原电位、电子光谱以及构效关系， Sandro，并通过分析这两种方法所带来的变化， 研究组设计了一对氨酰-tRNA合成酶/tRNA体系，瑞士苏黎世大学Deliz Liang。

Lau，尽管使用经典氨基酸可以在一定程度上实现疏水调控， particularly the turnover frequency and total turnover number. To understand this improved functionality，但引入非天然氨基酸能进一步扩展这种能力， we demonstrate the utility of hydrophobic tuning based on non-canonical amino acids (ncAAs) to engineer a bacterial laccase， the redox potentials，从而赋予新的或改进的功能，为了理解这种功能改进，创刊于2009年，最新IF：24.274 官方网址： https://www.nature.com/nchem/ 投稿链接： https://mts-nchem.nature.com/cgi-bin/main.plex ， Deliz Liang，。

本期文章：《自然—化学》：Online/在线发表 近日， Natter Perdiguero，隶属于施普林格自然出版集团， electronic spectra and structurefunction relationships were examined. Combining traditional directed evolution with ncAA-based engineering resulted in further improvements in catalysis， Alexandria团队研究了铜金属酶中非规范氨基酸的疏水调谐， 疏水性控制着蛋白质和酶功能的诸多方面。

所获得的突变显著提升了催化活性， Anton。